Dihydrofolate Reductase 抗体 (AA 1-187)
Quick Overview for Dihydrofolate Reductase 抗体 (AA 1-187) (ABIN5540915)
抗原
See all Dihydrofolate Reductase (DHFR) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-187
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纯化方法
- Protein-A affinity chromatography
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免疫原
- Recombinant human DHFR (1-187aa) purified from E. coli.
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亚型
- IgG1
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应用备注
- The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Liquid. In Phosphate-Buffered Saline ( pH 7.4) with 0.02 % Sodium Azide, 10 % Glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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有效期
- 12 months
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- Dihydrofolate Reductase (DHFR)
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别名
- dhfr,dhfrp1
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背景
- Dihydrofolate reductase (DHFR) catalyzes the DPH-dependent reduction of dihydrofolate to tetrahydrofolate, and is a crucial enzyme for the synthesis of purines, pyrimidines and some amino acids. Inhibition of the activity of this enzyme leads to arrest of D synthesis and cell death. Gene expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive.
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UniProt
- P00374
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途径
- Mitotic G1-G1/S Phases
抗原
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