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Dihydrofolate Reductase 抗体 (AA 1-187)

This 小鼠 单克隆 antibody specifically detects Dihydrofolate Reductase in WB 和 EIA. It exhibits reactivity toward 人.
产品编号 ABIN5540915
发货至: 中国
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Quick Overview for Dihydrofolate Reductase 抗体 (AA 1-187) (ABIN5540915)

抗原

See all Dihydrofolate Reductase (DHFR) 抗体
Dihydrofolate Reductase (DHFR)

适用

  • 63
  • 34
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  • 1
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宿主

  • 58
  • 8
  • 1
小鼠

克隆类型

  • 44
  • 23
单克隆

标记

  • 35
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  • 1
  • 1
  • 1
  • 1
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This Dihydrofolate Reductase antibody is un-conjugated

应用范围

  • 43
  • 27
  • 15
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  • 1
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Western Blotting (WB), Enzyme Immunoassay (EIA)

克隆位点

AT5B2
  • 抗原表位

    • 7
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
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    AA 1-187

    纯化方法

    Protein-A affinity chromatography

    免疫原

    Recombinant human DHFR (1-187aa) purified from E. coli.

    亚型

    IgG1
  • 应用备注

    The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Liquid. In Phosphate-Buffered Saline ( pH 7.4) with 0.02 % Sodium Azide, 10 % Glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

    有效期

    12 months
  • 抗原

    Dihydrofolate Reductase (DHFR)

    别名

    dhfr,dhfrp1

    背景

    Dihydrofolate reductase (DHFR) catalyzes the DPH-dependent reduction of dihydrofolate to tetrahydrofolate, and is a crucial enzyme for the synthesis of purines, pyrimidines and some amino acids. Inhibition of the activity of this enzyme leads to arrest of D synthesis and cell death. Gene expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive.

    UniProt

    P00374

    途径

    Mitotic G1-G1/S Phases
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