IL-10 抗体
Quick Overview for IL-10 抗体 (ABIN5540435)
抗原
See all IL-10 (IL10) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- This antibody detects Human Interleukin 10. Other species not tested.
-
纯化方法
- Affinity Chromatography on Protein A
-
免疫原
- Highly pure (> 98%) E.coli derived Recombinant Human IL-10 (Cat.-No PA084).
-
亚型
- IgG1
-
-
anti-Interleukin 10 (IL10) (AA 101-178) antibody
IL10 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) (AA 19-178) antibodyIL10 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) (AA 19-178) antibodyIL10 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) antibodyIL10 适用: 人, 小鼠, 大鼠, Cow, 兔, Pig, 马, 犬, 猴, 小鸡, 山羊, 绵羊, 豚鼠, Avian, Cat, 驴, Hamster WB 宿主: 山羊 Polyclonal unconjugated
anti-Interleukin 10 (IL10) antibodyMicroarray verified IL10 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB971550 unconjugated
anti-Interleukin 10 (IL10) antibodyMicroarray verified IL10 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB312926 unconjugated
anti-Interleukin 10 (IL10) antibodyIL10 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Interleukin 10 (IL10) (AA 27-53) antibodyIL10 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) (AA 1-178) antibodyIL10 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 (IL10) (AA 1-178) antibodyIL10 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C5 unconjugated
-
-
应用备注
- Sandwich ELISA (assuming 100 μL/well), a concentration of 8.0-10.0 μg/mL of this antibody will detect at least 1000 pg/well of recombinant Human IL-10 when used with Biotinylated Antigen Affinity purified anti-Human IL-10 (Cat.-No PP1032B) as the detection antibody at a concentration of approximately 0.05-0.1 μg/mL. Western Blot: To detect hIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions. Immunohistochemistry on Paraffin Sections: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 5.0 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
-
限制
- 仅限研究用
-
-
-
缓冲液
- PBS without preservatives
-
储存液
- Without preservative
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
-
有效期
- 12 months
-
-
- IL-10 (IL10) (Interleukin 10 (IL10))
-
别名
- interleukin-10,il10
-
背景
- Interleukin 10 (IL-10) is a 178 amino acid protein that is primarily secreted by TH2 clones. IL-10 has dual functions, the first of which is the suppression of cytokine production by TH1 clones responding to antigen presented by monocyte and macrophage antigen presenting cells (APCs). The second function consists of the inhibition of response of cytokine targeted cells, possibly by the downregulation of CD25 (the interleukin-2 receptor) on macrophages and B lymphocytes. IL-10 exerts its biological activity through the IL-10 receptor (IL-10R) a 560 amino acid, 110 kDa glycoprotein whose expression can be induced in cultured macrophages and fibroblasts by elevated in HIV-1 infected individuals and has been implicated in the progression of the disease.
-
途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
抗原
-
