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IL-7 抗体

The 兔 多克隆 anti-IL-7 antibody is suitable to detect IL-7 in samples from 大鼠. It has been validated for WB, Neut 和 EIA.
产品编号 ABIN5540412
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for IL-7 抗体 (ABIN5540412)

抗原

See all IL-7 (IL7) 抗体
IL-7 (IL7) (Interleukin 7 (IL7))

适用

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大鼠

宿主

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克隆类型

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多克隆

标记

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This IL-7 antibody is un-conjugated

应用范围

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Western Blotting (WB), Neutralization (Neut), Enzyme Immunoassay (EIA)
  • 特异性

    This antibody recognizes Rat IL7. Other species not tested.

    纯化方法

    Affinity Chromatography employing immobilized Human IL7 matrix

    免疫原

    Highly pure (> 98%) E.coli derived recombinant Rat IL7 (Cat.-No PA266)
  • 应用备注

    Sandwich ELISA: To detect Rat IL-7 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IL-7 (Cat.-No AP32852BT-N or AP32852BT-S) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-7. Western Blot: To detect Rat IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Neutralization: To yield one-half maximal inhibition [ND 50 ] of the biological activity of Rat IL-7 (5.0 ng/mL), a concentration of 0.14-0.21 μg/mL of this antibody is required.

    限制

    仅限研究用
  • 缓冲液

    PBS, pH 7.2

    储存条件

    4 °C,-20 °C

    储存方法

    Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

    有效期

    12 months
  • 抗原

    IL-7 (IL7) (Interleukin 7 (IL7))

    别名

    interleukin-7,il7

    背景

    IL-7 is a hematopoietic growth factor which affects primarily early B and T cells. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as ConA and IL-2. Human and murine IL-7 is cross-species reactive.

    途径

    JAK/STAT Signaling
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