SIGLEC-5,SIGLEC-14 抗体 (PE)
Quick Overview for SIGLEC-5,SIGLEC-14 抗体 (PE) (ABIN5540043)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognises human SIGLEC5 / CD170. Clone 1A5 antibody is one of several SIGLEC5 antibodies which also recognises human SIGLEC14 (Angata, T. et al., 2006).
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纯化方法
- Affinity chromatography on Protein G
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免疫原
- SIGLEC5-Fc protein, consisting of the full-length extracellular region of human SIGLEC5, fused with the Fc region of human IgG1
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亚型
- IgG1
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anti-SIGLEC-5,SIGLEC-14 (Internal Region) antibody
适用: 人 ELISA, IHC (p), WB 宿主: 兔 Polyclonal unconjugated
anti-SIGLEC-5,SIGLEC-14 antibody (PE)适用: 人, Chimpanzee FACS 宿主: 小鼠 Monoclonal 1A5 PE
anti-SIGLEC-5,SIGLEC-14 (Internal Region) antibody适用: 人 ELISA, IHC (p), WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Flow cytometry: Neat, use 10 μL to label 10e6 cells in 100 μL.
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限制
- 仅限研究用
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Prior to and following reconstitution store the antibody at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Shelf life: one year from despatch.
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有效期
- 12 months
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- SIGLEC-5,SIGLEC-14
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别名
- siglec5,siglec14
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背景
- Siglec-5 shares an almost identical sequence with Siglec-14 within the first two Ig-like domains, indicating partial gene conversion between these two Siglecs, also evident in other primate species. Siglec-5, also known as CD170, is a sialic-acid-binding Ig-like lectin, and member of the Ig superfamily, expressed by dendritic cells (DCs), activated macrophages, neutrophils, and cells of the monocyte/myeloid lineage. Highly related to the myelomonocytic-derived adhesion molecule CD33 (Siglec-3), Siglec-5 mediates sialic-acid dependent binding to cells, and is as well acting as an inhibitory receptor in the down-regulation of cell activation. Structurally, Siglec-5 contains an immunoreceptor tyrosine-based inhibitor motif (ITIM), which plays a part in the modulation of cellular responses, and when phosphorylated, can bind to the SH2 domain of several SH2-containing phosphatases. Siglec-14 is a putative sialic-acid binding adhesion molecule and predominantly expressed in hematopoietic tissues, which have been shown to associate with the activating adapter protein DAP12.
抗原
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