Intestinal Alkaline Phosphatase 抗体 (Biotin)
Quick Overview for Intestinal Alkaline Phosphatase 抗体 (Biotin) (ABIN5539993)
抗原
See all Intestinal Alkaline Phosphatase (ALPI) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This product detects bovine Alkaline phosphatase. Cross reactivity against Alkaline phosphatase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-rabbit serum as well as purified and partially purified Alkaline phosphatase [calf intestine].
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纯化方法
- Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
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免疫原
- Alkaline phosphatase from calf intestine
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应用备注
- Western Blot : 1/500-1/2,000. ELISA : 1/4,000-1/20,000. This antibody has been assayed against 1.0 μg of Alkaline phosphatase in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested.
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限制
- 仅限研究用
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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有效期
- 12 months
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- Intestinal Alkaline Phosphatase (ALPI)
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别名
- alkaline phosphatase,alpi,iap
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UniProt
- P19111
抗原
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