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Intestinal Alkaline Phosphatase 抗体

This 山羊 多克隆 anti-Intestinal Alkaline Phosphatase antibody specifically detects Intestinal Alkaline Phosphatase in WB, IP, EIA 和 DB. The antibody is reactive with Cow samples.
产品编号 ABIN5539990
发货至: 中国
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Quick Overview for Intestinal Alkaline Phosphatase 抗体 (ABIN5539990)

抗原

See all Intestinal Alkaline Phosphatase (ALPI) 抗体
Intestinal Alkaline Phosphatase (ALPI)

适用

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Cow

宿主

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山羊

克隆类型

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多克隆

标记

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This Intestinal Alkaline Phosphatase antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB)
  • 特异性

    This antibody detects bovine alkaline phosphatase. Cross reactivity against alkaline phosphatase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis gives a single precipitin arc against anti-Goat Serum as well as purified and partially purified alkaline phosphatase [calf intestine]. Species: Bovine.

    纯化方法

    Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer

    免疫原

    Alkaline phosphatase from calf intestine

    亚型

    IgG
  • 应用备注

    ELISA : 1/4000-1/20000. Western Blot : 1/500-1/2000. Immunoblotting (Western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.

    限制

    仅限研究用
  • 缓冲液

    0.01M Sodium phosphate, 0.14M Sodium chloride, pH 7.4

    储存条件

    4 °C,-20 °C

    储存方法

    Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

    有效期

    12 months
  • 抗原

    Intestinal Alkaline Phosphatase (ALPI)

    别名

    alkaline phosphatase,alpi,iap

    背景

    Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH . Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

    UniProt

    P19111
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