DS WB Results: Approx 30 kDa band observed in lysates of cell line Jurkat (calculated MW of 30.2 kDa according to NP_001165694.1). Recommended concentration: 0.02-0.06 μg/mL. An additional band of unknown identity was also consistently observed at 48 kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
DS IHC Results: In paraffin embedded Human Lung shows staining of smooth muscle cells, vasculature and apical secretory granules in the epithelium of the bronchiole. Recommended concentration, 5-10 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:128000.