ESR2 抗体 (AA 417-428)
Quick Overview for ESR2 抗体 (AA 417-428) (ABIN5539801)
抗原
See all ESR2 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 417-428
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原理
- Estrogen receptor beta (aa417-428)
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序列
- TQDADSSRKL AH
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特异性
- This antibody is expected to recognize all reported isoforms (NP_001428.1, NP_001035365.1, NP_001201831.1, NP_001201832.1). Reported variants represent identical protein: NP_001035365.1, NP_001035366.1
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交叉反应
- 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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亚型
- IgG
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应用备注
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Western Blot: Approx 50 kDa band observed in Human Placenta lysates (calculated MW of 53.0 kDa according to NP_001035366.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:128000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the nucleus of HeLa and MCF7 cells. Recommended concentration: 10μg/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- ESR2 (Estrogen Receptor 2 (ESR2))
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别名
- ESR2
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背景
- ESR2, estrogen receptor 2 (ER beta), ER-BETA, ESR-BETA, ESRB, ESTRB, Erb, NR3A2, OTTHUMP00000180647, estrogen nuclear receptor beta variant a, estrogen nuclear receptor beta variant b, estrogen receptor beta, estrogen receptor beta 4, nuclear receptor sub
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基因ID
- 2100
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NCBI登录号
- NP_001428, NP_001035365, NP_001201831, NP_001201832
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途径
- Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling
抗原
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