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Fibrillarin 抗体 (AA 99-110)

This anti-Fibrillarin antibody is a 山羊 多克隆 antibody detecting Fibrillarin in WB 和 ELISA. Suitable for 人.
产品编号 ABIN5539729
发货至: 中国

Quick Overview for Fibrillarin 抗体 (AA 99-110) (ABIN5539729)

抗原

See all Fibrillarin (FBL) 抗体
Fibrillarin (FBL)

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This Fibrillarin antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA

质量等级

Verified
  • 抗原表位

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    AA 99-110

    原理

    fibrillarin / FBL (aa99-110)

    序列

    CRGKEDALVT KN

    交叉反应

    Cow, 犬, 人, 小鼠, Pig, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 35 kDa band observed in lysates of cell line HEK293 (calculated MW of 33.8 kDa according to NP_001427.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:128000.

    说明

    Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    Fibrillarin (FBL)

    别名

    FBL

    背景

    FBL, fibrillarin, FIB, FLRN, RNU3IP1, 34 kDa nucleolar scleroderma antigen, 34-kD nucleolar scleroderma antigen, RNA, U3 small nucleolar interacting protein 1, rRNA 2'-O-methyltransferase fibrillarin

    基因ID

    2091, 14113, 292747

    NCBI登录号

    NP_001427

    途径

    Ribonucleoside Biosynthetic Process
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