GAPDH 抗体 (Internal Region) (Biotin)
Quick Overview for GAPDH 抗体 (Internal Region) (Biotin) (ABIN5539725)
抗原
See all GAPDH 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- GAPDH (Internal), Biotinylated
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序列
- GVNHEKYDNS LK.
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特异性
- This antibody is expected to recognize both reported isoforms (NP_002037.2, NP_001243728.1). Reported variants represent identical protein: NP_001276674.1, NP_002037.2, NP_001276675.1. GAPDH is constitutively expressed in almost all tissues at high levels
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交叉反应
- 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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亚型
- IgG
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应用备注
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Western Blot: Approx 36 kDa band observed in Human Liver lysates (calculated MW of 36.1 kDa according to NP_002037.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:16000.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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别名
- GAPDH
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背景
- GAPDH, glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD, HEL-S-162eP, aging-associated gene 9 protein, epididymis secretory sperm binding protein Li 162eP, peptidyl-cysteine S-nitrosylase GAPDH
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基因ID
- 2597, 14433, 24383
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NCBI登录号
- NP_002037, NP_001243728
抗原
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