GAPDH 抗体 (C-Term) (Biotin)
Quick Overview for GAPDH 抗体 (C-Term) (Biotin) (ABIN5539724)
抗原
See all GAPDH 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
 - C-Term
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原理
 - GAPDH (C Terminus) Loading Control, biotinylated
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序列
 - HQVVSSDFNS DT.
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特异性
 - GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
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交叉反应
 - 犬, 人, 小鼠, Pig
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纯化方法
 - Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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亚型
 - IgG
 
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应用备注
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Western Blot: Approx 35 kDa band observed in lysates of cel line HEK293 (calculated MW of 36.1 kDa according to NP_002037.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:64000.
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限制
 - 仅限研究用
 
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状态
 - Liquid
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浓度
 - 0.5 mg/mL
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缓冲液
 - Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
 - Sodium azide
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注意事项
 - This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
 - Minimize freezing and thawing.
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储存条件
 - -20 °C
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储存方法
 - Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
 
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- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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别名
 - GAPDH
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背景
 - GAPDH, glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD, HEL-S-162eP, aging-associated gene 9 protein, epididymis secretory sperm binding protein Li 162eP, peptidyl-cysteine S-nitrosylase GAPDH
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基因ID
 - 2597
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NCBI登录号
 - NP_002037
 
抗原
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