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C5AR1 抗体 (C-Term)

This anti-C5AR1 antibody is a 山羊 多克隆 antibody detecting C5AR1 in WB, FACS, ELISA, IHC 和 IF. Suitable for 人.
产品编号 ABIN5539670
发货至: 中国

Quick Overview for C5AR1 抗体 (C-Term) (ABIN5539670)

抗原

See all C5AR1 抗体
C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This C5AR1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

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    C-Term

    原理

    C5AR1/CD88

    序列

    RESKSFTRST VDT

    交叉反应

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 6-8 μg/mL.

    Western Blot: Approx 38 kDa band observed in lysates of cell line HepG2 (calculated MW of 39.3 kDa according to NP_001727.1). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:64000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/m

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

    别名

    C5AR1

    背景

    C5AR1, C5A, C5AR, C5A-R, C5R1, CD88, C5a anaphylatoxin chemotactic receptor, C5a anaphylatoxin receptor, Complement component 5 receptor 1, Complement component 5a receptor 1, Anaphylatoxin receptor

    基因ID

    728, 113959

    NCBI登录号

    NP_001727

    途径

    Complement System
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