SIGLEC5 抗体 (C-Term)
Our Local Distributor
北京 101111
Quick Overview for SIGLEC5 抗体 (C-Term) (ABIN5534705)
抗原
See all SIGLEC5 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 482-513, C-Term
-
纯化方法
- This antibody is purified through a protein A column, followed by peptide affinity purification.
-
免疫原
- This SIGLEC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 482-513 amino acids from the C-terminal region of human SIGLEC5.
-
亚型
- Ig Fraction
-
-
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 17-230) antibody
SIGLEC5 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 3F5A3 unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) antibodySIGLEC5 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (Extracellular Domain) antibody (APC)SIGLEC5 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 1A5 APC
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 147-335) antibodySIGLEC5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (N-Term) antibodySIGLEC5 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 452-551) antibodySIGLEC5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 458-551) antibody (Biotin)SIGLEC5 适用: 人 WB 宿主: 兔 Polyclonal Biotin
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (Extracellular Domain) antibodySIGLEC5 适用: 人, Non-Human Primate ELISA, IP, ICC, FACS, IHC (fro) 宿主: 小鼠 Monoclonal 1A5 unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (Extracellular Domain) antibody (PE)SIGLEC5 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 1A5 PE
anti-Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 18-135) antibodySIGLEC5 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- For WB starting dilution is: 1:1000
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.5 mg/mL
-
缓冲液
- Supplied in PBS with 0.09 % (W/V) sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
-
-
- SIGLEC5 (Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5))
-
别名
- SIGLEC5
-
背景
- SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have one or more cytoplasmic immune receptor tyrosine-based inhibitory motifs (ITIM). SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6. Sequence analysis predicted that the 697-amino acid SIGLEC10 protein contains a signal peptide, an N-terminal V-set Ig-like domain and four C2-set Ig-like domains, five potential N-linked glycosylation sites, a transmembrane region, and a 126-residue cytoplasmic tail with 3 putative ITIMs. Northern blot analysis detected a major 3.0-kb SIGLEC10 transcript, with highest levels in spleen, lymph node, blood leukocytes, and appendix. Little or no expression was observed in pancreas, thyroid, and testis. Flow cytometric analysis demonstrated eosinophil-specific expression of SIGLEC10, but at a lower level than that of SIGLEC8. Expression was also detected on monocytes and a CD16-positive/CD56-negative natural killer-like lymphocyte population. After sialidase treatment, which is necessary for unmasking the sialic acid-binding site on SIGLECs interacting with cell surface sialic acids, cells expressing SIGLEC10 bound to red blood cells. Immunoprecipitation analysis indicated expression of a 100- to 120-kD monomeric protein, higher than the predicted molecular mass, suggesting that SIGLEC10 is glycosylated.
-
分子量
- 61 kDa
-
基因ID
- 8778
-
UniProt
- O15389
抗原
-
