Protein Kinase A beta (AA 50-80), (N-Term), (Regulatory Subunit 1) 抗体
适用: 人, 小鼠
WB, IHC (p)
宿主: 兔
Polyclonal
unconjugated
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- 抗原
- Protein Kinase A beta
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抗原表位
- AA 50-80, N-Term, Regulatory Subunit 1
- 适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
- 免疫原
- This Protein Kinase A regulatory subunit I beta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-80 amino acids from the N-terminal region of human Protein Kinase A regulatory subunit I beta.
- 亚型
- Ig Fraction
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anti-Protein Kinase A beta (AA 50-80), (N-Term), (Regulatory Subunit 1) antibody
适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB03249 unconjugated
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- 应用备注
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For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 2 mg/mL
- 缓冲液
- Supplied in PBS with 0.09 % (W/V) sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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- 抗原
- Protein Kinase A beta
- 背景
- The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ, and Cγ, that each represent specific gene products. Cα, and Cβ, are closely related (93 % amino acid sequence similari- ty), whereas Cγ, displays 83 % and 79 % similarity to Cα, and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcrip- tion factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.
- 分子量
- 43 kDa
- 基因ID
- 5575
- UniProt
- P31321
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