CDC20 抗体
Quick Overview for CDC20 抗体 (ABIN5518976)
抗原
See all CDC20 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-Cdc20 Antibody Picoband®
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序列
- QTPTKKEHQK AWALNLNGFD VEEAKILRLS GKPQNAPEGY QNRLKVLYSQ KAT
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Cdc20 Antibody Picoband® (ABIN5518976). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human Cdc20, which shares 98.1% amino acid (aa) sequence identity with both mouse and rat Cdc20.
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亚型
- IgG
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anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (AA 1-270) antibody
CDC20 适用: 人 WB, IF, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) antibodyCDC20 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (AA 1-270) antibodyCDC20 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (AA 57-84) antibodyCDC20 适用: 人 WB, FACS, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) antibodyCDC20 适用: 人 FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal AR12 unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (N-Term) antibodyCDC20 适用: 人, 小鼠, 大鼠, Pig, 马, 豚鼠, 猴, Cow, 非洲爪蟾, Bat, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (AA 1-499) antibodyCDC20 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (N-Term) antibodyCDC20 适用: 人, 小鼠, 大鼠 WB, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (AA 57-84), (N-Term) antibodyCDC20 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB23122 unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) antibodyCDC20 适用: 人, 小鼠, 大鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells
1. Herzog, F., Primorac, I., Dube, P., Lenart, P., Sander, B., Mechtler, K., Stark, H., Peters, J.-M. Structure of the anaphase-promoting complex/cyclosome interacting with a mitotic checkpoint complex. Science 323: 1477-1481, 2009. 2. Morris, M. C., Kaiser, P., Rudyak, S., Baskerville, C., Watson, M. H., Reed, S. I. Cks1-dependent proteasome recruitment and activation of CDC20 transcription in budding yeast. Nature 423: 1009-1013, 2003. 3. Reddy, S. K., Rape, M., Margansky, W. A., Kirschner, M. W. Ubiquitination by the anaphase-promoting complex drives spindle checkpoint inactivation. Nature 446: 921-925, 2007. 4. Yang, Y., Kim, A. H., Yamada, T., Wu, B., Bilimoria, P. M., Ikeuchi, Y., de la Iglesia, N., Shen, J., Bonni, A. A Cdc20-APC ubiquitin signaling pathway regulates presynaptic differentiation. Science 326: 575-578, 2009. -
说明
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We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))
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别名
- CDC20
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背景
- Cell division cycle protein 20 homolog, p55CDC, CDC20,
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分子量
- 55 kDa
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基因ID
- 991
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UniProt
- Q12834
抗原
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