COPE 抗体 (AA 80-308)
Quick Overview for COPE 抗体 (AA 80-308) (ABIN5518902)
抗原
See all COPE 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 80-308
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原理
- Anti-COPE Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-COPE Antibody Picoband® (ABIN5518902). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human COPE recombinant protein (Position: E80-A308). Human COPE shares 89.5% amino acid (aa) sequence identity with mouse COPE.
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亚型
- IgG
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anti-Coatomer Protein Complex, Subunit epsilon (COPE) (AA 211-308) antibody
COPE 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Coatomer Protein Complex, Subunit epsilon (COPE) (AA 2-308) antibodyCOPE 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Coatomer Protein Complex, Subunit epsilon (COPE) antibodyCOPE 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Coatomer Protein Complex, Subunit epsilon (COPE) (AA 1-308) antibodyCOPE 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Coatomer Protein Complex, Subunit epsilon (COPE) (AA 1-308) antibodyCOPE 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Coatomer Protein Complex, Subunit epsilon (COPE) (AA 273-300), (C-Term) antibodyCOPE 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB19580 unconjugated
anti-Coatomer Protein Complex, Subunit epsilon (COPE) (AA 280-308), (C-Term) antibodyCOPE 适用: 人 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Coatomer Protein Complex, Subunit epsilon (COPE) antibodyCOPE 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. "Entrez Gene: COPE coatomer protein complex, subunit epsilon". 2. Shima DT, Scales SJ, Kreis TE, Pepperkok R (November 1999). "Segregation of COPI-rich and anterograde-cargo-rich domains in endoplasmic-reticulum-to-Golgi transport complexes". Curr. Biol. 9 (15): 821-4. 3. Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksöz E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H, Wanker EE (September 2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957-68. -
说明
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We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- COPE (Coatomer Protein Complex, Subunit epsilon (COPE))
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别名
- COPE
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背景
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Synonyms: Coatomer subunit epsilon,Epsilon-coat protein,Epsilon-COP,COPE,
Tissue Specificity: Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon.
Background: Coatomer subunit epsilon is a protein that in humans is encoded by the COPE gene. The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.
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分子量
- 34 kDa
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基因ID
- 11316
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UniProt
- O14579
抗原
-
