Calpain 2 抗体 (AA 500-700)
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- 抗原 See all Calpain 2 (CAPN2) 抗体
- Calpain 2 (CAPN2)
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抗原表位
- AA 500-700
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Calpain 2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
- 原理
- Anti-Calpain 2/CAPN2 Antibody Picoband®
- 交叉反应 (详细)
- No cross-reactivity with other proteins.
- 产品特性
- Anti-Calpain 2/CAPN2 Antibody Picoband® (ABIN5518897). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- 纯化方法
- Immunogen affinity purified.
- 免疫原
- E. coli-derived human Calpain 2 recombinant protein (Position: R500-L700). Human Calpain 2 shares 93.5% and 92.5% amino acid (aa) sequence identity with mouse and rat Calpain 2, respectively.
- 亚型
- IgG
- Top Product
- Discover our top product CAPN2 Primary Antibody
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- 应用备注
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Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat,
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Hata A, Ohno S, Akita Y, Suzuki K (May 1989). "Tandemly reiterated negative enhancer-like elements regulate transcription of a human gene for the large subunit of calcium-dependent protease". J. Biol. Chem. 264 (11): 6404-11. 2. Imajoh S, Aoki K, Ohno S, Emori Y, Kawasaki H, Sugihara H, Suzuki K (May 1989). "Molecular cloning of the cDNA for the large subunit of the high-Ca2+-requiring form of human Ca2+-activated neutral protease". Biochemistry. 27 (21): 8122-8. - 说明
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We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- 抗原
- Calpain 2 (CAPN2)
- 别名
- CAPN2 (CAPN2 产品)
- 背景
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Synonyms: Calpain-2 catalytic subunit,3.4.22.53,Calcium-activated neutral proteinase 2,CANP 2,Calpain M-type,Calpain large polypeptide L2,Calpain-2 large subunit,Millimolar-calpain,M-calpain,CAPN2,CANPL2,
Tissue Specificity: Ubiquitous.
Background: Calpain-2 catalytic subunit is a protein that in humans is encoded by the CAPN2 gene. The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported.
- 分子量
- 80 kDa
- 基因ID
- 824
- UniProt
- P17655
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