PDE5A 抗体 (N-Term)
Quick Overview for PDE5A 抗体 (N-Term) (ABIN5518785)
抗原
See all PDE5A 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 20-63, N-Term
-
原理
- Anti-PDE5A Antibody Picoband®
-
序列
- QKQQQRDQDS VEAWLDDHWD FTFSYFVRKA TREMVNAWFA ERVH
-
交叉反应 （详细）
- No cross-reactivity with other proteins
-
产品特性
- Anti-PDE5A Antibody Picoband® (ABIN5518785). Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human PDE5A, different from the related mouse sequence by eight amino acids.
-
亚型
- IgG
-
-
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) (C-Term) antibody
Verified PDE5A 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) (AA 29-138) antibodyPDE5A 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 9H5 unconjugated
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) (C-Term) antibodyPDE5A 适用: 人, 大鼠, 小鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) (AA 101-200) antibodyPDE5A 适用: 人, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) (C-Term) antibodyPDE5A 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-phosphodiesterase 5A, cGMP-Specific (PDE5A) (AA 1-150) antibodyPDE5A 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Gebska, M. A., Stevenson, B. K., Hemnes, A. R., Bivalacqua, T. J., Haile, A., Hesketh, G. G., Murray, C. I., Zaiman, A. L., Halushka, M. K., Krongkaew, N., Strong, T. D., Cooke, C. A., El-Haddad, H., Tuder, R. M., Berkowitz, D. E., Champion, H. C.Phosphodiesterase-5A (PDE5A) is localized to the endothelial caveolae and modulates NOS3 activity. Cardiovasc. Res. 90: 353-363, 2011. 2. Lin, C.-S., Chow, S., Lau, A., Tu, R., Lue, T. F. Human PDE5A gene encodes three PDE5 isoforms from two alternate promoters.Int. J. Impotence Res. 14: 15-24, 2002. 3. Loughney, K., Hill, T. R., Florio, V. A., Uher, L., Rosman, G. J., Wolda, S. L., Jones, B. A., Howard, M. L., McAllister-Lucas, L. M., Sonnenburg, W. K., Francis, S. H., Corbin, J. D., Beavo, J. A., Ferguson, K. Isolation and characterization of cDNAs encodingPDE5A, a human cGMP-binding, cGMP-specific 3-prime,5-prime-cyclic nucleotide phosphodiesterase. Gene 216: 139-147, 1998. -
说明
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
-
-
- PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))
-
别名
- PDE5A
-
背景
-
Synonyms: cGMP-specific 3',5'-cyclic phosphodiesterase,3.1.4.35 ,cGMP-binding cGMP-specific phosphodiesterase,CGB-PDE,PDE5A,PDE5,
Tissue Specificity: Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.
Background: cGMP-specific phosphodiesterase type 5 is an enzyme from the phosphodiesterase class. It is found in various tissues, most prominently the corpus cavernosum and the retina. It has also been recently discovered to play a vital role in the cardiovascular system. Furthermore, PDE5A also plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This PDE5A gene is mapped to 4q26.
-
分子量
- 100 kDa
-
基因ID
- 8654
-
UniProt
- O76074
-
途径
- Regulation of G-Protein Coupled Receptor Protein Signaling
抗原
-
