Nanog 抗体 (Middle Region)
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北京 101111
Quick Overview for Nanog 抗体 (Middle Region) (ABIN5518779)
抗原
See all Nanog (NANOG) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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原理
- Anti-Nanog Antibody
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序列
- QRQKYLSLQQ MQELSNILNL SYKQVKTWFQ NQRMKSKRWQ K
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特异性
- No cross reactivity with other proteins.
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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预测反应
- different from the related mouse sequence by three amino acids.
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产品特性
- Anti-Nanog Antibody. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human Nanog, different from the related mouse sequence by three amino acids.
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亚型
- IgG
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应用备注
- Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
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说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- Nanog (NANOG) (Nanog Homeobox (NANOG))
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别名
- NANOG
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背景
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Background: NANOG (pron. nanOg) is a transcription factor critically involved with self-renewal of undifferentiated embryonic stem cells. In humans, this protein is encoded by the NANOG gene. It is mapped to 12p13.31. NANOG is thought to be a key factor in maintaining pluripotency. Moreover, NANOG is also thought to function in concert with other factors such as POU5F1 (Oct-4) and SOX2 to establish ESC identity. The < a href=https://www.bosterbio.com/human-nanog-recombinant-protein-protq9h9s0-boster.html style=color:#EA8D28>NANOG protein has been found to be a transcriptional activator for the Rex1 promoter, playing a key role in sustaining Rex1 expression. Knockdown of NANOG in embryonic stem cells results in a reduction of Rex1 expression, while forced expression of NANOG stimulates Rex1 expression.
Gene Full Name: Nanog homeobox
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分子量
- 48 kDa
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基因ID
- 79923
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UniProt
- Q9H9S0
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途径
- Stem Cell Maintenance
抗原
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