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HMGB1 抗体 (C-Term)

Name: HMGB1 抗体 (C-Term)
SKU: ABIN5518759
Availability: OutOfStock

Cited in 4+ publications. This anti-HMGB1 antibody is a 兔多克隆 antibody detecting HMGB1 in WB, IHC, IF, ICC 和 FACS. Suitable for 人, 小鼠和大鼠.

Western blot analysis of HMGB1 expression in rat brain extract ( Lane 1), mouse ovary extract ( Lane 2), 22RV1 whole cell lysates ( Lane 3) and HELA whole cell lysates ( Lane 4). (HMGB1 抗体 (C-Term))

HMGB1 was detected in paraffin-embedded sections of mouse liver tissues using rabbit anti- HMGB1 Antigen Affinity purified polyclonal antibody (Catalog # ) at 1 µg/mL. (HMGB1 抗体 (C-Term))

IHC

HMGB1 was detected in paraffin-embedded sections of rat intestine tissues using rabbit anti- HMGB1 Antigen Affinity purified polyclonal antibody (Catalog # ) at 1 µg/mL. (HMGB1 抗体 (C-Term))

IHC

HMGB1 was detected in paraffin-embedded sections of rat liver tissues using rabbit anti- HMGB1 Antigen Affinity purified polyclonal antibody (Catalog # ) at 1 µg/mL. (HMGB1 抗体 (C-Term))

7 images

(4 references)

产品编号 ABIN5518759

发货至: 中国

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Quick Overview for HMGB1 抗体 (C-Term) (ABIN5518759)

抗原

See all HMGB1 抗体

HMGB1 (High Mobility Group Box 1 (HMGB1))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This HMGB1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Discover our top product HMGB1 Primary Antibody

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AA 124-154, C-Term

原理

Anti-HMGB1 Antibody

序列

DVAKKLGEMW NNTAADDKQP YEKKAAKLKE K

交叉反应（详细）

No cross-reactivity with other proteins

产品特性

Anti-HMGB1 Antibody (ABIN5518759). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human HMGB1, identical to the related mouse and rat sequences.

亚型

IgG
anti-High Mobility Group Box 1 (HMGB1) (AA 75-170) antibody
HMGB1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-High Mobility Group Box 1 (HMGB1) (AA 1-90) antibody
HMGB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2F6 unconjugated

anti-High Mobility Group Box 1 (HMGB1) antibody
KO Validated HMGB1 适用: 人 WB, IHC, IF, ICC, IHC (p), IHC (wm), IHC (ff) 宿主: 兔 Polyclonal unconjugated

anti-High Mobility Group Box 1 (HMGB1) (AA 9-163) antibody
HMGB1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibody
HMGB1 适用: 小鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibody
HMGB1 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibody
HMGB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1D5 unconjugated

anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibody
HMGB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1E6-E10 unconjugated

anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibody
HMGB1 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal C4 unconjugated

anti-High Mobility Group Box 1 (HMGB1) (N-Term) antibody
HMGB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Jia, Sun, Hu, Li, Ruan: "Propofol inhibits the release of interleukin-6, 8 and tumor necrosis factor-α correlating with high-mobility group box 1 expression in lipopolysaccharides-stimulated RAW 264.7 cells." in: BMC anesthesiology, Vol. 17, Issue 1, pp. 148, (2018) (PubMed).

Ruisong, Xiaorong, Gangying, Chunfeng, Changjiang, Xuefei, Yuanhong, Hong: "The Protective Role of Interleukin-33 in Myocardial Ischemia and Reperfusion Is Associated with Decreased HMGB1 Expression and Up-Regulation of the P38 MAPK Signaling Pathway." in: PLoS ONE, Vol. 10, Issue 11, pp. e0143064, (2016) (PubMed).

Li, Zhou, Zhou, Zou: "Galantamine protects against lipopolysaccharide-induced acute lung injury in rats." in: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, Vol. 49, Issue 2, pp. e5008, (2016) (PubMed).

Bi, Zhu, Yan, Chen, Wang, Ma, Yang: "Association of Upregulated HMGB1 and c-IAP2 Proteins With Hepatocellular Carcinoma Development and Progression." in: Hepatitis monthly, Vol. 14, Issue 12, pp. e23552, (2015) (PubMed).
抗原

HMGB1 (High Mobility Group Box 1 (HMGB1))

别名

HMGB1

背景

Synonyms: High mobility group protein B1,High mobility group protein 1,HMG-1,HMGB1,HMG1,
Tissue Specificity: Ubiquituous. Expressed in platelets (PubMed:11154118). .
Background: High mobility group box 1 protein, also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a protein that in humans is encoded by the HMGB1 gene. This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.

分子量

25 kDa

基因ID

3146

UniProt

P09429

途径

p53 Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome