Sulfotyrosine 抗体 (Atto 594)
Quick Overview for Sulfotyrosine 抗体 (Atto 594) (ABIN5067491)
抗原
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine.
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纯化方法
- Protein G Purified
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免疫原
- Synthetic Sulfotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG1
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anti-Sulfotyrosine antibody
ELISA, ICC, IF, IHC, WB 宿主: 小鼠 Monoclonal 7C5 unconjugated
anti-Sulfotyrosine antibody (HRP)ELISA, ICC, IF, IHC, WB 宿主: 小鼠 Monoclonal 7C5 HRP
anti-Sulfotyrosine antibody (FITC)ELISA, ICC, IF, IHC, WB 宿主: 小鼠 Monoclonal 7C5 FITC
anti-Sulfotyrosine antibody (Biotin)ELISA, ICC, IF, IHC, WB 宿主: 小鼠 Monoclonal 7C5 Biotin
anti-Sulfotyrosine antibody (PE)ELISA, ICC, IF, IHC, WB 宿主: 小鼠 Monoclonal 7C5 PE
anti-Sulfotyrosine antibody (APC)ELISA, ICC, IF, IHC, WB 宿主: 小鼠 Monoclonal 7C5 APC
anti-Sulfotyrosine antibody (PerCP)ELISA, ICC, IF, IHC, WB 宿主: 小鼠 Monoclonal 7C5 PerCP
anti-Sulfotyrosine antibody (Atto 488)ELISA, ICC, IF, IHC, WB 宿主: 小鼠 Monoclonal 7C5 Atto 488
anti-Sulfotyrosine antibody (Atto 390)ELISA, ICC, IF, IHC, WB 宿主: 小鼠 Monoclonal 7C5 Atto 390
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应用备注
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- WB (1:1000)
- ICC/IF (1:50)
- ELISA (1:1000)
- IHC (1:25)
- optimal dilutions for assays should be determined by the user.
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说明
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A 1:1000 dilution of ABIN5067491 was sufficient for detection of Sulfotyrosine in 0.5 μg of Sulfotyrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- Sulfotyrosine
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物质类
- Amino Acid
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背景
- Tyrosine sulfation is a common post-translational protein modification catalyzed by tyrosylprotein sulfotransferase (TPST) (1). Sulfotyrosines mediate interactions between hirudin and thrombin, P-selectin glycoprotein ligand-1 and P-selectin, and von Willebrand factor and factor VIII. Tyrosine sulfation of certain receptors is required for several viruses and parasites to enter their hosts.
抗原
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