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Sulfotyrosine 抗体 (HRP)

ELISA, ICC, IF, WB, IHC 宿主: 小鼠 Monoclonal 7C5 HRP
产品编号 ABIN5067500
发货至: 中国
  • 抗原 See all Sulfotyrosine products
    Sulfotyrosine
    宿主
    • 10
    小鼠
    克隆类型
    • 10
    单克隆
    标记
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Sulfotyrosine antibody is conjugated to HRP
    应用范围
    ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC)
    特异性
    Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine.
    纯化方法
    Protein G Purified
    免疫原
    Synthetic Sulfotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
    克隆位点
    7C5
    亚型
    IgG1
  • 应用备注
    • WB (1:1000)
    • ICC/IF (1:50)
    • ELISA (1:1000)
    • IHC (1:25)
    • optimal dilutions for assays should be determined by the user.
    说明

    A 1:1000 dilution of ABIN5067500 was sufficient for detection of Sulfotyrosine in 0.5 μg of Sulfotyrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Conjugated antibodies should be stored at 4°C
  • 抗原
    Sulfotyrosine
    Abstract
    Sulfotyrosine 产品
    物质类
    Amino Acid
    背景
    Tyrosine sulfation is a common post-translational protein modification catalyzed by tyrosylprotein sulfotransferase (TPST) (1). Sulfotyrosines mediate interactions between hirudin and thrombin, P-selectin glycoprotein ligand-1 and P-selectin, and von Willebrand factor and factor VIII. Tyrosine sulfation of certain receptors is required for several viruses and parasites to enter their hosts.
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