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Hexanoyl-Lysine Adduct (HEL) 抗体 (PE)

This anti- antibody is a 小鼠 单克隆 antibody detecting in ELISA, WB, FACS, ICC 和 IF. Suitable for 所有种类.
产品编号 ABIN5067251
发货至: 中国

Quick Overview for Hexanoyl-Lysine Adduct (HEL) 抗体 (PE) (ABIN5067251)

抗原

Hexanoyl-Lysine Adduct (HEL)

适用

所有种类

宿主

  • 10
小鼠

克隆类型

  • 10
单克隆

标记

  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
PE

应用范围

  • 10
  • 10
  • 9
  • 9
  • 9
  • 1
  • 1
ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

克隆位点

5D9
  • 特异性

    Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.

    纯化方法

    Protein G Purified

    免疫原

    Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH).

    亚型

    IgG1
  • 应用备注

    • WB (1:1000)
    • ICC/IF (1:50)
    • ELISA (1:1000)
    • FACS (1:50)
    • FCM (1:50)
    • optimal dilutions for assays should be determined by the user.

    说明

    A 1:1000 dilution of ABIN5067251 was sufficient for detection of Hexanoyl Lysine adduct in 0.5 μg of Hexanoyl Lysine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C
  • 抗原

    Hexanoyl-Lysine Adduct (HEL)

    别名

    Hexanoyl-Lysine adduct

    背景

    Hexanoyl-lysine adduct (HEL) is a lysine adduct of 13-HPODE and is produced by the oxidation of omega-6 polyunsaturated fatty acids (1). It is a biomarker for the initial stage of lipid peroxidation. Lipid peroxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging.
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