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Hexanoyl-Lysine Adduct (HEL) 抗体 (Biotin)

适用: 所有种类 ELISA, WB, FACS, ICC, IF 宿主: 小鼠 Monoclonal 5D9 Biotin
产品编号 ABIN5067246
发货至: 中国
  • 抗原
    Hexanoyl-Lysine Adduct (HEL)
    适用
    所有种类
    宿主
    • 10
    小鼠
    克隆类型
    • 10
    单克隆
    标记
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Biotin
    应用范围
    • 10
    • 10
    • 9
    • 9
    • 9
    • 1
    • 1
    ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
    特异性
    Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
    纯化方法
    Protein G Purified
    免疫原
    Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH).
    克隆位点
    5D9
    亚型
    IgG1
  • 应用备注
    • WB (1:1000)
    • ICC/IF (1:50)
    • ELISA (1:1000)
    • FACS (1:50)
    • FCM (1:50)
    • optimal dilutions for assays should be determined by the user.
    说明

    A 1:1000 dilution of ABIN5067246 was sufficient for detection of Hexanoyl Lysine adduct in 0.5 μg of Hexanoyl Lysine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
  • 抗原
    Hexanoyl-Lysine Adduct (HEL)
    别名
    Hexanoyl-Lysine adduct
    背景
    Hexanoyl-lysine adduct (HEL) is a lysine adduct of 13-HPODE and is produced by the oxidation of omega-6 polyunsaturated fatty acids (1). It is a biomarker for the initial stage of lipid peroxidation. Lipid peroxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging.
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