Hexanoyl-Lysine Adduct (HEL) 抗体 (FITC)
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- 抗原
- Hexanoyl-Lysine Adduct (HEL)
- 适用
- 所有种类
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- FITC
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应用范围
- ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
- 特异性
- Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
- 纯化方法
- Protein G Purified
- 免疫原
- Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH).
- 克隆位点
- 5D9
- 亚型
- IgG1
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- 应用备注
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- WB (1:1000)
- ICC/IF (1:50)
- ELISA (1:1000)
- FACS (1:50)
- FCM (1:50)
- optimal dilutions for assays should be determined by the user.
- 说明
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A 1:1000 dilution of ABIN5067247 was sufficient for detection of Hexanoyl Lysine adduct in 0.5 μg of Hexanoyl Lysine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
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- 抗原
- Hexanoyl-Lysine Adduct (HEL)
- 别名
- Hexanoyl-Lysine adduct
- 背景
- Hexanoyl-lysine adduct (HEL) is a lysine adduct of 13-HPODE and is produced by the oxidation of omega-6 polyunsaturated fatty acids (1). It is a biomarker for the initial stage of lipid peroxidation. Lipid peroxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging.
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