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GPI 抗体

This anti-GPI antibody is a 小鼠 单克隆 antibody detecting GPI in WB. Suitable for Sudan ebolavirus.
产品编号 ABIN5027226
发货至: 中国

Quick Overview for GPI 抗体 (ABIN5027226)

抗原

See all GPI 抗体
GPI (Glucose-6-Phosphate Isomerase (GPI))

适用

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Sudan ebolavirus

宿主

  • 97
  • 26
  • 1
小鼠

克隆类型

  • 89
  • 35
单克隆

标记

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This GPI antibody is un-conjugated

应用范围

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Western Blotting (WB)

克隆位点

ABM47F9
  • 纯化方法

    Protein G Chromatography

    免疫原

    A partial Length recombinant GP I protein of Sudan Ebola virus was used as an immunogen for this antibody.

    亚型

    IgG2b kappa
  • 应用备注

    WB: 0.5-1 μg/mL

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    PBS containing 0.05 % BSA, PH 7.4

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
  • 抗原

    GPI (Glucose-6-Phosphate Isomerase (GPI))

    别名

    GP I

    背景

    The Sudan ebola virus (SUDV) glycoprotein (GP) is an envelope glycoprotein that is present on the virion surface and is involved in receptor binding and mediating viral entry. It is composed of a trimer of heterodimers (GP1/GP2), where GP1 and GP2 remain covalently linked by a disulfide bond9, and the resulting GP1-GP2 pair trimerizes to form a ~450 kDa envelope spike on the viral surface. GP is synthesized as a single polypeptide of 676 amino acids in length that is post-translationally cleaved by furin to yield two subunits, GP1 and GP2. The GP1 subunit contains two heavily glycosylated domains, the glycan cap and the mucin-like domain (MLD). The glycan cap contains only N-linked glycans, whereas the MLD contains both N- and O-linked glycans. All 15 N-glycosylation sites of GP1 could be removed without compromising the expression of GP. In the endosome, a flexible loop containing GP1 residues 190 213 is cleaved by host cathepsins. This cleavage releases the glycan cap and mucin-like domains from GP1. The GP1 subunit is responsible for receptor binding and attachment to new host cells.

    分子量

    50 kDa

    基因ID

    3160774

    UniProt

    Q7T9D9
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