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GAPDH 抗体 (AA 120-320)

This anti-GAPDH antibody is a 小鼠 单克隆 antibody detecting GAPDH in WB 和 IHC. Suitable for 人.
产品编号 ABIN5027214
发货至: 中国

Quick Overview for GAPDH 抗体 (AA 120-320) (ABIN5027214)

抗原

See all GAPDH 抗体
GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This GAPDH antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC)

克隆位点

ABM22C5
  • 抗原表位

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    AA 120-320

    交叉反应

    小鼠

    纯化方法

    Protein G Chromatography

    免疫原

    A partial length recombinant GAPDH protein (amino acids 120-320) was used as the immunogen for this antibody.

    亚型

    IgG1 kappa
  • 应用备注

    WB: 0.5-1 μg/mL, IHC: 5 μg/mL

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    PBS containing 0.05 % BSA, PH 7.4

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
  • 抗原

    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

    别名

    GAPDH

    背景

    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) is an enzyme best known for its role in glycolysis. However, extra-glycolytic functions of GAPDH have been described, including regulation of protein expression via RNA binding. GAPDH binds to numerous AREs (adenine-uridine rich elements) from various mRNA 3'-untranslated regions in vitro and in vivo despite its lack of a canonical RNA binding motif. GAPDH specifically catalyzes the simultaneous phosphorylation and oxidation of glyceraldehyde 3-phosphate using NAD+ (Nicotinamide Adenine Dinucleotide) as a cofactor to produce glycerate 1,3-biphosphate and NADH. In addition to its role in energy production, GAPDH has been implicated in many cellular processes including DNA repair tRNA export, membrane fusion and transport, endocytosis and nuclear membrane assembly, and cell death.

    分子量

    36 kDa

    基因ID

    2597

    UniProt

    P04406
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