SKA1 抗体 (C-Term)
Quick Overview for SKA1 抗体 (C-Term) (ABIN500728)
抗原
See all SKA1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- This antibody is specific to SKA1 and will not cross-react with SKA2 or SKA3.
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纯化方法
- Peptide Afiinity Chromatography
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免疫原
- SKA1 antibody was raised against a 13 amino acid peptide from near the carboxy terminus of Human SKA1.
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亚型
- IgG
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anti-Spindle and Kinetochore Associated Complex Subunit 1 (SKA1) (C-Term) antibody
SKA1 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Spindle and Kinetochore Associated Complex Subunit 1 (SKA1) (AA 61-134) antibodySKA1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Spindle and Kinetochore Associated Complex Subunit 1 (SKA1) (AA 51-100) antibodySKA1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA. Western blot: 0.5 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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缓冲液
- PBS containing 0.02 % Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- SKA1 (Spindle and Kinetochore Associated Complex Subunit 1 (SKA1))
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别名
- SKA1
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背景
- Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintenance of the metaphase plate and spindle checkpoint silencing.Synonyms: C18orf24, Spindle and kinetochore-associated protein 1
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基因ID
- 220134
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UniProt
- Q96BD8
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途径
- M Phase
抗原
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