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Nephrin 抗体 (C-Term)

Name: Nephrin 抗体 (C-Term)
SKU: ABIN500330
Availability: OutOfStock

There are 2+ publications for this product available. The 兔多克隆 anti-Nephrin antibody is suitable to detect Nephrin in samples from 人, 小鼠和大鼠. It has been validated for WB, IF, IHC (p) 和 EIA.

Western blot analysis in mouse kidney tissue lysate using Nephrin Antibody at 1 μg/ml in the (A) absence and (B) presence of blocking peptide. (Nephrin 抗体 (C-Term))

Immunofluorescence in mouse kidney cells using Nephrin Antibody at 10 µg/ml. (Nephrin 抗体 (C-Term))

Immunohistochemistry in mouse kidney tissue using Nephrin Antibody at 1 μg/ml. (Nephrin 抗体 (C-Term))

IHC (p)

3 images

(2 references)

产品编号 ABIN500330

发货至: 中国

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Quick Overview for Nephrin 抗体 (C-Term) (ABIN500330)

抗原

See all Nephrin (NPHS1) 抗体

Nephrin (NPHS1) (Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This Nephrin antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Discover our top product NPHS1 Primary Antibody

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C-Term

特异性

This antibody detects Nephrin.

纯化方法

Peptide affinity chromatography

免疫原

Nephrin antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human Nephrin.

亚型

IgG
anti-Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1) (AA 23-92) antibody
NPHS1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1) (AA 451-550) antibody
NPHS1 适用: 人, 小鼠, 大鼠, 犬 FACS 宿主: 兔 Polyclonal unconjugated

anti-Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1) (AA 27-234) antibody
NPHS1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1) (AA 27-234) antibody
NPHS1 适用: 人 WB, ICC, IF 宿主: 小鼠 Monoclonal C8 unconjugated

anti-Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1) (AA 23-257) antibody
NPHS1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1) (AA 1191-1219), (Tyr1210) antibody
NPHS1 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB28264 unconjugated

anti-Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1) (AA 39-144) antibody
NPHS1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1) (AA 39-106) antibody
NPHS1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated

anti-Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1) (AA 33-122) antibody
NPHS1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3D2 unconjugated

anti-Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1) (pTyr1217) antibody
NPHS1 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

PBS containing 0.02 % Sodium azide

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Gu, Zhou, Noble, Border, Cheung, Huang: "Targeting reduction of proteinuria in glomerulonephritis: Maximizing the antifibrotic effect of valsartan by protecting podocytes." in: Journal of the renin-angiotensin-aldosterone system : JRAAS, Vol. 15, Issue 2, pp. 177-89, (2015) (PubMed).

Zhang, Gu, Lawrence, Cheung, Huang: "A plasminogen activator inhibitor type 1 mutant retards diabetic nephropathy in db/db mice by protecting podocytes." in: Experimental physiology, Vol. 99, Issue 5, pp. 802-15, (2014) (PubMed).
抗原

Nephrin (NPHS1) (Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1))

别名

Nephrin

背景

Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm. Despite its predicted molecular weight, Nephrin often migrates at a lower than expected size in SDS-PAGE.Synonyms: NPHN, NPHS1

基因ID

4868

NCBI登录号

NP_004637

UniProt

O60500

途径

Regulation of Actin Filament Polymerization, Skeletal Muscle Fiber Development