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GAPDH 抗体

This anti-GAPDH antibody is a 小鼠 单克隆 antibody detecting GAPDH in WB, IHC (p) 和 FACS. Suitable for 人, 小鼠, 大鼠 和 Pig. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN5003194
发货至: 中国

Quick Overview for GAPDH 抗体 (ABIN5003194)

抗原

See all GAPDH 抗体
GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

适用

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人, 小鼠, 大鼠, Pig

宿主

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小鼠

克隆类型

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单克隆

标记

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This GAPDH antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

克隆位点

4F8
  • 交叉反应

    人, 小鼠, Pig, 大鼠

    纯化方法

    Purified by Protein G.

    免疫原

    KLH conjugated synthetic peptide derived from human GAPDH

    亚型

    IgG
  • 应用备注

    WB 1:300-5000
    FCM 1:20-100
    IHC-P 1:200-400

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 μg/μL

    缓冲液

    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

    有效期

    12 months
  • Cui, Hao, Li, Bao, Li, Gao, Gu: "Chronic Heat Stress Induces Immune Response, Oxidative Stress Response, and Apoptosis of Finishing Pig Liver: A Proteomic Approach." in: International journal of molecular sciences, Vol. 17, Issue 5, (2017) (PubMed).

  • 抗原

    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

    别名

    GAPDH

    背景

    Synonyms: Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, GAPD, CDABP0047, OK/SW-cl.12

    Background: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

    基因ID

    2597

    UniProt

    P04406
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