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DFFA 抗体 (N-Term)

Name: DFFA 抗体 (N-Term)
SKU: ABIN499744
Availability: OutOfStock

DFFA 适用: 人 WB, EIA 宿主: 兔 Polyclonal unconjugated

Western blot analysis of DFF45 in HeLa cell lysate with AP30290PU-N DFF45 antibody at (A) 1 and (B) 2 μg/ml. (DFFA 抗体 (N-Term))

1 image

产品编号 ABIN499744

发货至: 中国

Datasheet as PDF

抗原 See all DFFA 抗体

DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
抗原表位
15

14

9

8

6

6

5

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
适用
73

33

20

2

1

1

1

1
人
宿主
75

9

1
兔
Compatible Secondaries
克隆类型
77

8
多克隆
标记
61

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1
This DFFA antibody is un-conjugated
应用范围
82

30

15

14

13

13

12

10

6

5

3

2

1

1

1

1
Western Blotting (WB), Enzyme Immunoassay (EIA)

特异性

DFF45 antibody was raised against a peptide corresponding to amino acids 2 to 21 of human DFF45.

纯化方法

DEAE

免疫原

Human DFF45 (N-Terminus) Peptide

亚型

IgG

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anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 1-331) antibody
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anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 2-21) antibody
DFFA 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

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DFFA 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 2B10C8 unconjugated

anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 19-262) antibody
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DFFA 适用: 人, 猴 WB, IF 宿主: 小鼠 Monoclonal 3F12 unconjugated

anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (C-Term) antibody
DFFA 适用: 人 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA. Western Blot: DFF45 antibody can be used for detection of DFF45, DFF35, and one thecleaved fragment at 1/1000 to 1/2000 dilution.45 and 35 kDa bands can be detected innon-apoptotic cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
缓冲液

PBS containing 0.02 % sodium azide.

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store the antibody undiluted at 2-8 °C.
抗原

DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

别名

DFFA / ICAD (DFFA 产品)

别名

DFF-45 antibody, DFF1 antibody, ICAD antibody, A330085O09Rik antibody, DFF35 antibody, Dff45 antibody, ICAD-L antibody, ICAD-S antibody, DFFA antibody, zgc:92229 antibody, DNA fragmentation factor subunit alpha antibody, DNA fragmentation factor, alpha subunit antibody, DNA fragmentation factor, 45kDa, alpha polypeptide antibody, DNA fragmentation factor, alpha polypeptide antibody, DFFA antibody, Dffa antibody, dffa antibody

背景

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently that was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD. DFF45/ICAD have short forms that were termed DFF35 and ICADs, respectively. Upon cleavage of DFF45/ICAD, the caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.Synonyms: DFF-45, DFF1, DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor subunit alpha, Inhibitor of CAD

基因ID

1676

NCBI登录号

NP_004392

UniProt

O00273

途径

Apoptosis, Caspase Cascade in Apoptosis