DFFA 抗体 (C-Term)
Quick Overview for DFFA 抗体 (C-Term) (ABIN499742)
抗原
See all DFFA 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- DFF45 antibody was raised against a peptide corresponding to amino acids 313 to 331 of human DFF45.
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纯化方法
- DEAE
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免疫原
- Human DFF45 (C-Terminus) Peptide
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亚型
- IgG
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anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 1-331) antibody
DFFA 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 1-331) antibodyDFFA 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 51-305) antibodyDFFA 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 2-21) antibodyDFFA 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 1-331) antibodyDFFA 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 231-331) antibodyDFFA 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 3A11 unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 179-331) antibodyDFFA 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 2B10C8 unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 19-262) antibodyDFFA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) antibodyDFFA 适用: 人, 猴 WB, IF 宿主: 小鼠 Monoclonal 3F12 unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (C-Term) antibodyDFFA 适用: 人 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA. Western Blot: DFF45 antibody can be used for detection of DFF45 and one of the cleavedfragments of DFF45 at 1/1000 to 1/2000 dilution. A 45 kDa band can be detected innon-apoptotic cells. Immunocytochemistry. Immunoprecipitation: DFF45 antibody can be used for detection of DFF45 and one of thecleaved fragments of DFF45 at 1/1000 to 1/2000 dilution.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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缓冲液
- PBS containing 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C.
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- DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
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别名
- DFFA / ICAD
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背景
- Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD (2,3). Upon cleavage of DFF45/ICAD, a caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.Synonyms: DFF-45, DFF1, DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor subunit alpha, Inhibitor of CAD
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基因ID
- 1676
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NCBI登录号
- NP_004392
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UniProt
- O00273
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途径
- Apoptosis, Caspase Cascade in Apoptosis
抗原
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