LIG1 抗体 (Internal Region)
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Quick Overview for LIG1 抗体 (Internal Region) (ABIN499175)
抗原
See all LIG1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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序列
- C-RVREDKQPEQ ATTS
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特异性
- This antibody detects DNA ligase 1 (Internal).
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交叉反应 （详细）
- Species reactivity (expected):RatSpecies reactivity (tested):Human
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纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from the internal region (near C-Terminus) of the protein sequence according to NP_000225.1
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anti-Ligase I, DNA, ATP-Dependent (LIG1) antibody
LIG1 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Ligase I, DNA, ATP-Dependent (LIG1) (AA 810-919) antibodyLIG1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal AG12 unconjugated
anti-Ligase I, DNA, ATP-Dependent (LIG1) (AA 565-790) antibodyLIG1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ligase I, DNA, ATP-Dependent (LIG1) (Internal Region) antibodyLIG1 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ligase I, DNA, ATP-Dependent (LIG1) antibodyLIG1 适用: 人 WB, IHC, ELISA 宿主: 兔 Monoclonal 7B2 unconjugated Recombinant Antibody
anti-Ligase I, DNA, ATP-Dependent (LIG1) (AA 810-920) antibodyLIG1 适用: 人 WB, IHC, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 10G12 unconjugated
anti-Ligase I, DNA, ATP-Dependent (LIG1) (AA 1-919) antibodyLIG1 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Ligase I, DNA, ATP-Dependent (LIG1) antibodyLIG1 适用: 人, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Ligase I, DNA, ATP-Dependent (LIG1) antibodyLIG1 适用: 人, 大鼠, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ligase I, DNA, ATP-Dependent (LIG1) (C-Term) antibodyLIG1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Peptide ELISA: 1: 16000 (limit dilution). Western blot: 0.1-0.3 μg/mL. Approx 125 kDa band observed in nuclear lysates of cell lineHeLa (calculated MW of 105 kDa according to NP_000225.1). The observed molecularweight corresponds to earlier findings with different antibodies from other commercialsources.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C up to one week or (in aliquots) at -20 °C for longer.
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- LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))
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别名
- DNA Ligase 1
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背景
- Eukaryotic DNA ligases are ATP-dependent enzymes that catalyse the joining of single and double-strand DNA breaks, which is an essential final step in DNA replication, recombination and repair. Four biochemically distinct DNA ligases, termed ligases I-IV, have been identified in mammalian cells. DNA ligase I is functionally homologous to the DNA ligase encoded by the Saccharomyces cerevisiae Cdc9 gene. The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is due to DNA ligase I.Synonyms: DNA ligase 1, DNA ligase I, EC=6.5.1.1, LIG-1, LIG1, Polydeoxyribonucleotide synthase [ATP] 1
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基因ID
- 3978
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NCBI登录号
- NP_000225
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UniProt
- P18858
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途径
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
抗原
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