CST8 抗体 (Internal Region)
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Quick Overview for CST8 抗体 (Internal Region) (ABIN499119)
抗原
See all CST8 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 100-112, Internal Region
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序列
- STNEICAIQE NSK
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特异性
- This antibody recognizes Cystatin 8 (aa 100-112)
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交叉反应 (详细)
- Species reactivity (tested):Human.
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纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence from the internal region of the protein sequence according to NP_005483.1.
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应用备注
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Peptide ELISA: Detection Limit: 1/16000. Western blot: 1-3 μg/mL. In transfected HEK293 transiently expressing Human CST8 (MYCand DYKDDDDK tagged), a band of approx.23 kDa is observed. No bands are observed inmock-transfected HEK293 and the same band is observed using anti-myc tag antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CST8 (Cystatin 8 (CST8))
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别名
- Cystatin 8
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背景
- The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein similar to type 2 cystatins. CST8 performs a specialized role during sperm development and maturation.Synonyms: CRES, CST8, Cystatin-related epididymal spermatogenic protein
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基因ID
- 10047
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NCBI登录号
- NP_005483
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UniProt
- O60676
抗原
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