ME1 抗体
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北京 101111
Quick Overview for ME1 抗体 (ABIN499096)
抗原
See all ME1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of Malic enzyme 1 protein. (region surrounding Tyr500)
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交叉反应 （详细）
- Species reactivity (expected):Mouse and RatSpecies reactivity (tested):Human.
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纯化方法
- Affinity chromatography using epitope-specific immunogen
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纯度
- > 95 % pure by SDS-PAGE
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anti-Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 1-572) antibody
ME1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3H5 unconjugated
anti-Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) antibodyKO Validated ME1 适用: 人 WB, IF, IHC (p), ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 161-519) antibodyME1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 1-572) antibodyME1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 1-572) antibodyME1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2A2 unconjugated
anti-Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (C-Term) antibodyME1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (N-Term) antibodyME1 适用: 人, 小鼠, 大鼠, 犬, 兔, 马, Pig, 绵羊, Cow WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 37-70) antibodyME1 适用: 人 WB 宿主: 兔 Polyclonal RB55906 unconjugated
anti-Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 1-300) antibodyME1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1) (AA 36-85) antibodyME1 适用: 人, 犬, Pig WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot: 1: 500 - 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
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- ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))
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别名
- NADP-ME
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背景
- ME1 (Malic enzyme 1), also known as NADP-ME, MES or HUMNDME, is a 572 amino acid cytoplasmic protein that belongs to the malic enzyme family. Expressed ubiquitously with highest expression in liver and white adipose tissue, ME1 functions as an NADP-dependent enzyme that catalyzes the conversion of S-malate and NADP to pyruvate, carbon dioxide and NADPH (a reducing agent that participates in fatty acid biosynthesis). Through its ability to catalyze the reversible oxidative decarboxylation of malate, ME1 links the citric acid and glycolytic cycles. ME1 exists as a homotetramer that uses divalent metal cations, such as magnesium or manganese, as cofactors. The expression of ME1 is regulated by both thyroid hormone levels and the amount of carbohydrates in the diet, indicating that ME1 may play an important role as a housekeeping protein within the cell.Synonyms: ME1, NADP-dependent malic enzyme
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分子量
- approx. 64 kDa
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基因ID
- 4199
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NCBI登录号
- NP_002386
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UniProt
- P48163
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途径
- Regulation of Lipid Metabolism by PPARalpha
抗原
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