POLR3D 抗体
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北京 101111
Quick Overview for POLR3D 抗体 (ABIN499065)
抗原
See all POLR3D 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of POLR3D protein. (region surrounding asn314)
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交叉反应 （详细）
- Species reactivity (tested):Human, Mouse.
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纯化方法
- Affinity chromatography using epitope-specific immunogen
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纯度
- > 95 % (by SDS-PAGE)
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anti-Polymerase (RNA) III (DNA Directed) Polypeptide D, 44kDa (POLR3D) (AA 1-398) antibody
POLR3D 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide D, 44kDa (POLR3D) (AA 296-395) antibodyPOLR3D 适用: 人 ELISA 宿主: 小鼠 Monoclonal 4E6 unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide D, 44kDa (POLR3D) (Internal Region) antibodyPOLR3D 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide D, 44kDa (POLR3D) (AA 175-225) antibodyPOLR3D 适用: 人 WB, IP, IHC (fp) 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide D, 44kDa (POLR3D) (AA 348-398) antibodyPOLR3D 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide D, 44kDa (POLR3D) (AA 175-225) antibodyPOLR3D 适用: 人 IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide D, 44kDa (POLR3D) antibodyPOLR3D 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide D, 44kDa (POLR3D) (AA 127-156) antibodyPOLR3D 适用: 人 WB 宿主: 兔 Polyclonal RB33419 unconjugated
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应用备注
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Western blot: 1: 500 - 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
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- POLR3D (Polymerase (RNA) III (DNA Directed) Polypeptide D, 44kDa (POLR3D))
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别名
- POLR3D
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背景
- Three genes (POLR2 J1, POLR2 J2, and POLR2 J3) form a cluster of total length of 214 530 bp in the genetic locus 7q22.1 on the long arm of chromosome 7 (contig NT_007933). The fourth gene (POLR2 J4, 31 040 bp) was localized in the cytogenetic locus 7p13 of the short arm of chromosome 7 (contig NT_007819). An analysis enabled us to refine dissimilar experimental data on the mapping of the hRPB11 subunit gene on chromosome 7. In particular, the presence of three sites of its localization according to data on hybridization with fluorescent-labeled probes (the FISH method) was explained. It was established that, upon the expression of the four human POLR2 J genes, at least 14 types of mature mRNAs encoding somewhat differing hRPB11 isoforms can be synthesized. Eleven of these mRNAs were revealed (as full-length copies or clearly identifiable fragments) in the available databases of expressed sequence tags and cDNAs. The most probable scheme of origination of the multiple genes of the POLR2 J family as a result of three consecutive segmented duplications increasing in size was proposed and substantiated.Synonyms: BN51, BN51T, DNA-directed RNA polymerase III subunit D, DNA-directed RNA polymerase III subunit RPC4, RNA polymerase III 47 kDa subunit, RNA polymerase III subunit C4, RPC4, RPC53 homolog
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分子量
- approx. 38 kDa
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基因ID
- 661
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NCBI登录号
- NP_001713
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UniProt
- P05423
抗原
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