HLA-DOA 抗体
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北京 101111
Quick Overview for HLA-DOA 抗体 (ABIN498962)
抗原
See all HLA-DOA 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of HLADOalpha protein. (region surrounding Val111)
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Affinity chromatography
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纯度
- > 95 % by SDS-PAGE
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anti-HLA Class II DO alpha (HLA-DOA) (AA 1-250) antibody
HLA-DOA 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-HLA Class II DO alpha (HLA-DOA) (AA 26-135) antibodyHLA-DOA 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-HLA Class II DO alpha (HLA-DOA) (AA 1-250) antibodyHLA-DOA 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-HLA Class II DO alpha (HLA-DOA) antibodyHLA-DOA 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-HLA Class II DO alpha (HLA-DOA) antibodyHLA-DOA 适用: 人 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-HLA Class II DO alpha (HLA-DOA) (AA 31-59), (N-Term) antibodyHLA-DOA 适用: 人 WB 宿主: 兔 Polyclonal RB42412 unconjugated
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应用备注
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Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- HLA-DOA (HLA Class II DO alpha (HLA-DOA))
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别名
- HLA Class II DO alpha / HLA-DOA
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背景
- Reacts with HLA-DO, a heterodimer formed by DNalpha and DObeta subunits in B cells. DNalpha and DObeta are the products of the non-classical class II genes, HLA-DNA and HLA-DOB, respectively. DO forms tight complexes with DM in the endoplasmic reticulum and is thereby sorted to lysosomal vesicles during antigen processing and presentation. It is tightly associated with DM and it is selectively expressed on antigenpresenting cells, such as B cells, dendritic cells and thymic epithelial cells. DO can enhance the efficiency of peptide loading and has been found to stabilize DM at low pH , preserving its chaperon activity. DO-DM complexes are more efficient than DM in protecting empty DR molecules. Reports describe DO as a co-chaperone of DM. This antibody has also been reported to be suitable for western blot and immunoprecipitation.Synonyms: HLA class II histocompatibility antigen DO alpha chain, HLA-DNA, HLA-DZA, MHC DN-alpha, MHC DZ alpha, MHC class II antigen DOA
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分子量
- approx. 34 kDa
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基因ID
- 3111
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NCBI登录号
- NP_002110
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UniProt
- P06340
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途径
- Human Leukocyte Antigen (HLA) in Adaptive Immune Response
抗原
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