EIF4E 抗体 (pSer209)
Quick Overview for EIF4E 抗体 (pSer209) (ABIN498734)
抗原
See all EIF4E 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- pSer209
-
特异性
- This antibody detects endogenous levels of eIF4E protein only when phosphorylated at Ser209.
-
交叉反应 （详细）
-
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography
-
纯度
- > 95 % by SDS-PAGE
-
-
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 121-217) antibody
EIF4E 适用: 人, 大鼠 WB, ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 1-100) antibodyEIF4E 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) antibodyEIF4E 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 5D11 unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (pSer209) antibodyEIF4E 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (C-Term) antibodyEIF4E 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) antibodyEIF4E 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (pSer209) antibodyEIF4E 适用: 人, 大鼠, 小鼠, 斑马鱼 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (C-Term) antibodyEIF4E 适用: 人, 大鼠, 小鼠, 斑马鱼, Cow, 兔, 犬, 豚鼠, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (pSer209) antibodyEIF4E 适用: 人, 大鼠, 小鼠 WB, IHC (p), IF (cc) 宿主: 兔 Monoclonal 2B1 unconjugated
anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) (AA 1-217) antibodyEIF4E 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
浓度
- 1,0 mg/mL
-
缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C/-20 °C
-
储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
-
- EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))
-
别名
- EIF4E
-
背景
- The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4G, eIF4E, and eIF4A. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E is a 25 kDa protein that binds to the mRNA Synonyms: EIF-4E, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein
-
分子量
- approx. 25 kDa
-
基因ID
- 1977
-
NCBI登录号
- NP_001959
-
UniProt
- P06730
-
途径
- BCR Signaling
抗原
-
