Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
The effects of some oncogenes, growth factors and neuropeptides are mediated by tyrosine phosphorylation of focal adhesion kinase (FAK) and paxillin cytoskeletal proteins. A rapid increase in tyrosine phosphorylation of paxillin, FAK and Crk-associated substrate (CAS) are prominent early events triggered by many G protein-coupled receptors. In addition to G protein-coupled receptors, Angiotensin IV (Ang IV), protein kinase C and other proteins can also mediate the tyrosine phosphorylation of paxillin. Paxillin must bind FAK for maximal phosphorylation in response to cell adhesion. FAK may function to direct tyrosine phosphorylation of paxillin in the process of transformation by the Src oncogene.Synonyms: FLJ16691