Pepsin 抗体
The 兔 多克隆 anti-Pepsin antibody (ABIN498687) specifically detects Pepsin in WB 和 IHC (p).
The antibody is reactive with 人 samples.
产品编号 ABIN498687
发货至:
中国
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Quick Overview for Pepsin 抗体 (ABIN498687)
抗原
See all Pepsin 抗体
Pepsin
适用
人
宿主
兔
克隆类型
多克隆
标记
This Pepsin antibody is un-conjugated
应用范围
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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特异性
- This antibody detects endogenous levels of Pepsin protein. (region surrounding Met307)
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Affinity Chromatography using epitope-specific immunogen
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anti-Pepsin antibody
适用: 人, Pig, Hamster, 绵羊, 大鼠, 兔, 小鼠, 猴, 马, 豚鼠, 山羊, 驴, 犬, Cow, 小鸡, Cat, Avian WB 宿主: 山羊 Polyclonal unconjugated
anti-Pepsin (AA 352-381), (C-Term) antibody适用: 人 WB, EIA, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Pepsin
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背景
- Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor, pepsinogen, by removal of a 41 amino acid segment from the N-terminus. Pepsinogen is synthesized in the stomach lining, and hydrochloric acid, also produced by the gastric mucosa, is necessary to convert the inactive enzyme and to maintain the optimum acidity ( pH 1-3) for Pepsin function. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. Pepsin shows extremely broad specificity, although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. Pepsin A is a member of the subfamily A1 within the Pepsin family and is the predominant endopeptidase in the gastric juice of vertebrates. Pepsin A is inhibited by ovUS-1, a uterine Serpin.Synonyms: PGA, PGA3, PGA4, PGA5, Pepsin A, Pepsinogen A, pepsinogen 3 group I, pepsinogen 4 group I, pepsinogen 5 group I
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分子量
- approx. 42 kDa
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基因ID
- 5222
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NCBI登录号
- NP_001073276
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UniProt
- P00790
抗原
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