SIAH1 抗体
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北京 101111
Quick Overview for SIAH1 抗体 (ABIN498346)
抗原
See all SIAH1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of Siah-1/2 protein.
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交叉反应 （详细）
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Species reactivity (tested):Human, Mouse, Rat.
Also reacts to: SIAH2 -
纯化方法
- Affinity Chromatography using epitope-specific immunogen
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anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (N-Term) antibody
Verified SIAH1 适用: 人, 大鼠 WB, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 1-313) antibodySIAH1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 1-110) antibodySIAH1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C5 unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (N-Term) antibodySIAH1 适用: 人, 大鼠, 小鼠, 犬, 斑马鱼, Cow, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 191-240) antibodySIAH1 适用: 人, 大鼠, 小鼠, 犬, 斑马鱼, Cow, 豚鼠, 马, 兔, 非洲爪蟾, 小鸡, Bat, Hamster, 猴, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (N-Term) antibodySIAH1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 201-282) antibodySIAH1 适用: 大鼠, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (C-Term) antibodySIAH1 适用: 人, 大鼠, 小鼠, 犬, 斑马鱼, Cow, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 1-110) antibodySIAH1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2F26 unconjugated
anti-E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (N-Term) antibodySIAH1 适用: 人, 大鼠, 小鼠, 犬, 斑马鱼, Cow, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH ~7.2, 15 mM Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))
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别名
- SIAH1
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背景
- SIAH, the human homologue of the Drosophila seven in absentia (sina) gene, is a tumor suppressor protein that is expressed in intestinal epithelium and activated during apoptosis. Human SIAH proteins are produced as two distinct gene products, SIAH-1, and a slightly larger protein SIAH-2, which share a highly conserved C-terminal sequence and differ in their N-terminal regions. SIAH-1 is a 32 kDa protein that contains an N-terminal RING-finger domain, which is required for proteolysis, and a cystein-rich C-terminal domain, which regulates oligomerization and SIAH binding to target proteins. As a tumor suppressor, SIAH-1 binds DCC (deleted in colorectal cancer) and regulates DCC degradation via the ubiquitin-proteasome pathway. SIAH-1 also binds a Bcl-2 related protein, Bag-1, thereby inhibiting cell growth. The majority of SIAH-1 is localized to the nucleus, however a small percentage is detected in the cytoplasm. This nuclear localization suggests that SIAH proteins may interact with other nuclear matrix proteins and DNA.Synonyms: E3 ubiquitin-protein ligase SIAH1, HUMSIAH, Seven in absentia homolog 1, Siah-1, Siah-1a
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分子量
- approx. 40.0 kDa
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基因ID
- 6477
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NCBI登录号
- NP_001006611
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UniProt
- Q8IUQ4
抗原
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