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SIAH1 抗体 (N-Term)

Verified SIAH1 适用: 人, 大鼠 WB, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN184675
发货至: 中国
  • 抗原 See all SIAH1 抗体
    SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))
    抗原表位
    • 15
    • 7
    • 7
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    N-Term
    适用
    • 36
    • 24
    • 24
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    人, 大鼠
    宿主
    • 48
    • 2
    • 1
    山羊
    克隆类型
    • 49
    • 2
    多克隆
    标记
    • 19
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This SIAH1 antibody is un-conjugated
    应用范围
    • 42
    • 22
    • 13
    • 13
    • 6
    • 5
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF)
    原理
    SIAH1
    序列
    SRQTATALPT GTSKC
    特异性
    This product is expected to recognize both reported isoforms (NP_003022.3, NP_001006611.1)
    交叉反应
    Cow, 犬, 人, 小鼠, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence SRQTATALPTGTSKC, from the N Terminus of the protein sequence according to NP_003022.3, NP_001006611.1.
    亚型
    IgG
  • 应用备注

    Western Blot: Approx. 37 kDa band observed in Human Liver lysates and approx. 30+37 kDa bands in Rat Liver lysates (calculated MW of 34.6 kDa according to Human NP_001006611.1 and 31.1 kDa according to Rat NP_543181.2 and 38.2 kDa according to Rat XP_03895336

    Peptide ELISA: antibody detection limit dilution 1:128000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of U2OS and HepG2 cells. Recommended concentration: 10μg/ml.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Sroka, Voigt, Deeg, Reed, Schulz, Bhr, Kermer: "BAG1 modulates huntingtin toxicity, aggregation, degradation, and subcellular distribution." in: Journal of neurochemistry, Vol. 111, Issue 3, pp. 801-7, (2009) (PubMed).

  • 抗原
    SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))
    别名
    SIAH1 (SIAH1 产品)
    别名
    SIAH antibody, Siah1 antibody, Siah1a antibody, id:ibd2635 antibody, zgc:56026 antibody, SIAH1A antibody, siah antibody, E3 ubiquitin-protein ligase Siah1 antibody, E3 ubiquitin-protein ligase siah-1 antibody, siah E3 ubiquitin protein ligase 1 antibody, siah E3 ubiquitin protein ligase 1 L homeolog antibody, Tsp_03055 antibody, siah-1 antibody, Siah1 antibody, siah1 antibody, SIAH1 antibody, siah1.L antibody
    背景
    SIAH1, seven in absentia homolog 1 (Drosophila), Siah-1, hSIAH1, HUMSIAH, Siah-1a, FLJ08065, seven in absentia homolog 1, sonic hedgehog homolog
    分子量
    31.1kDa
    基因ID
    6477, 20437, 140941
    NCBI登录号
    NP_003022, NP_001006611
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