SKP1 抗体
Quick Overview for SKP1 抗体 (ABIN498169)
抗原
See all SKP1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of Skp1 p19 protein.
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography using epitope-specific immunogen
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纯度
- > 95 % (by SDS-PAGE)
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anti-S-Phase Kinase-Associated Protein 1 (SKP1) antibody
SKP1 适用: 人 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 4E11 unconjugated
anti-S-Phase Kinase-Associated Protein 1 (SKP1) (Internal Region) antibodySKP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-S-Phase Kinase-Associated Protein 1 (SKP1) (AA 53-160) antibodySKP1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1H8 unconjugated
anti-S-Phase Kinase-Associated Protein 1 (SKP1) (AA 1-163) antibodySKP1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-S-Phase Kinase-Associated Protein 1 (SKP1) (AA 1-163) antibodySKP1 适用: 人, 大鼠, 猴, 小鸡, Cow, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-S-Phase Kinase-Associated Protein 1 (SKP1) antibodySKP1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-S-Phase Kinase-Associated Protein 1 (SKP1) (AA 1-160) antibodySKP1 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-S-Phase Kinase-Associated Protein 1 (SKP1) (AA 81-163) antibodySKP1 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-S-Phase Kinase-Associated Protein 1 (SKP1) (AA 1-163) antibodySKP1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-S-Phase Kinase-Associated Protein 1 (SKP1) (AA 2-163) antibodySKP1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
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- SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))
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别名
- SKP1 / OCP2
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背景
- The critical role that the family of regulatory proteins known as cyclins plays in eukaryotic cell cycle regulation is well established. The best characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of MPF (maturation promoting factor). Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DP-1 and E2F and retinoblastoma protein p110. Two cyclin A-Cdk2 complex binding proteins, Skp1 p19 and Skp2 p45, have been described. Although the Skps (S phase kinase-associated proteins) associate with the active cyclin A-Cdk2 complex, they do not exhibit any regulatory effects on the complex. Abolition of Skp2 p45 function by either microinjection of anti-p45 antibodies or addition of antisense oligonucleotides prevents entry into S phase of both normal and transformed cells.Synonyms: Cyclin A/CDK2-associated protein p19, EMC19, OCP-2, OCP-II, Organ of Corti protein 2, RNA polymerase II elongation factor-like protein, S-phase kinase-associated protein 1, SKP1A, TCEB1L, Transcription elongation factor B, p19A, p19skp1
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分子量
- approx. 19 kDa
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基因ID
- 6500
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NCBI登录号
- NP_008861
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UniProt
- P63208
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途径
- Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases
抗原
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