RAD52 抗体
Our Local Distributor
北京 101111
Quick Overview for RAD52 抗体 (ABIN498166)
抗原
See all RAD52 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- This antibody detects endogenous levels of Rad52 protein.
-
交叉反应 （详细）
-
Species reactivity (expected):Mouse.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography using epitope-specific immunogen
-
纯度
- > 95 % (by SDS-PAGE)
-
-
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) (AA 269-418) antibody
RAD52 适用: 人 WB, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 5E11E7 unconjugated
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) (Internal Region) antibodyRAD52 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) (Thr56) antibodyRAD52 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) antibodyKD Validated RAD52 适用: 人 WB 宿主: 兔 Monoclonal 25GB3575 unconjugated Recombinant Antibody
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) antibodyRAD52 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) (AA 70-119) antibodyRAD52 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) antibodyRAD52 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) (AA 70-119), (pTyr104) antibodyRAD52 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
浓度
- 1.0 mg/mL
-
缓冲液
- Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C/-20 °C
-
储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
-
-
- RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))
-
别名
- RAD52
-
背景
- DNA double-strand breaks are generated by ionizing radiation and endogenously produced radicals, and they often are repaired through the RAD52 homologous recombination pathway. The RAD52 family includes RAD51, RAD52, RAD54, RAD54B and MRE11 genes. Rad51 and Rad52 proteins perform the key steps in homologous recombination (HR), including the search for DNA homology and strand exchange, through similar mechanisms. Mre11 functions in both non-homologous end joining, and meiotic HR, and it is essential in mitosis for chromosome maintenance. Rad54 belongs to the SWI2/SNF2 subfamily of ATPases, which includes the DNA helicases involved in replication, recombination, and repair, as it contains seven amino acid sequence motifs that are largely conserved. Rad54 ATPase activity is dependent on double-stranded (ds) DNA, and the ATPase activity of Rad54 is not absolutely required for its DNA repair function, suggesting that these activities occur at distinct regions of the molecule. RAD54B is significantly homologous to the RAD54 recombination gene. Expression of RAD54B is highest in testis and spleen, which are active in both meiotic and mitotic recombination.Synonyms: DNA repair protein RAD52 homolog
-
分子量
- approx. 46 kDa
-
基因ID
- 5893
-
NCBI登录号
- NP_602296
-
UniProt
- P43351
-
途径
- DNA Damage Repair
抗原
-
