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- 抗原 See all PPARA 抗体
- PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PPARA antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF)
- 特异性
- This antibody detects endogenous levels of PPAR-α protein.
- 交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - 纯化方法
- Affinity chromatography using epitope-specific immunogen
- 纯度
- > 95 % (by SDS-PAGE)
- Top Product
- Discover our top product PPARA Primary Antibody
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anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (AA 301-400) antibody
PPARA 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, FACS, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (AA 61-170) antibodyPPARA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (AA 1-120) antibodyPPARA 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 5D10C3 unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (AA 1-468) antibodyPPARA 适用: 人, 小鼠 WB, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal 1331CT894-186-143 unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (N-Term) antibodyKO Validated PPARA 适用: 人 WB, IP, IHC (p), ChIP 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (AA 1-120) antibodyPPARA 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 5D10E10 unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (C-Term) antibodyPPARA 适用: 人, 小鼠, 大鼠, 犬, 马, Cow, Pig, 山羊, 豚鼠, 兔, 绵羊, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (N-Term) antibodyPPARA 适用: 人, 小鼠 WB, ELISA, FACS, IHC, IF, FM 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (pSer12) antibodyPPARA 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (N-Term) antibodyPPARA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western blot: 1: 500 - 1: 1000. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
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- 抗原
- PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))
- 别名
- PPAR-alpha (PPARA 产品)
- 背景
- Peroxisome proliferators are nongenotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed Peroxisome Proliferator Activated Receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alph,a like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex.Synonyms: NR1C1, Nuclear receptor subfamily 1 group C member 1, PPAR, PPARA, Peroxisome proliferator-activated receptor alpha
- 分子量
- approx. 52 kDa
- 基因ID
- 5465
- NCBI登录号
- NP_001001928
- UniProt
- Q07869
- 途径
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C
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