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ACAT1 抗体 (Internal Region)

This 山羊 多克隆 antibody specifically detects ACAT1 in WB, IF 和 EIA. It exhibits reactivity toward 人, 小鼠, 大鼠 和 Cow.
产品编号 ABIN498064
发货至: 中国
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Quick Overview for ACAT1 抗体 (Internal Region) (ABIN498064)

抗原

See all ACAT1 抗体
ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

适用

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人, 小鼠, 大鼠, Cow

宿主

  • 77
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山羊

克隆类型

  • 65
  • 25
多克隆

标记

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This ACAT1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
  • 抗原表位

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    AA 257-269, Internal Region

    序列

    C-KRVDFSKVPK LKT

    特异性

    Recognizes Acetoacetyl Coenzyme A Thiolase/ACAT1 (257-269).

    交叉反应 (详细)

    Species reactivity (expected):Mouse, Rat, Bovine.
    Species reactivity (tested):Human.

    纯化方法

    Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

    免疫原

    Peptide from the internal region of Human ACAT1 according to NP_000010.1. Genename: ACAT1
  • 应用备注

    Peptide ELISA: Detection Limit: 1/8000. Western blot: 0.01-0.03 μg/mL. Detects a band of Approx 45 kDa in Human Liver lysates (calculated MW of 45.5 kDaaccording to NP_000010.1).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

    别名

    ACAT1 / MAT

    背景

    ACAT1, acetoacetyl CoA thiolase, plays a major role in ketone body metabolism. It is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.Synonyms: Acetoacetyl-CoA thiolase T2, Acetyl-CoA acetyltransferase mitochondrial

    基因ID

    38

    NCBI登录号

    NP_000010

    UniProt

    P24752
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