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NEFH 抗体

Name: NEFH 抗体
SKU: ABIN4956221
Availability: OutOfStock

This anti-NEFH antibody is a 小鸡多克隆 antibody detecting NEFH in WB, IHC, IF 和 ICC. Suitable for 人, 大鼠, Pig 和 Cow. This Primary Antibody has been cited in 1 publication.

CBB shows lane of Coomassie Brilliant Blue stained crude extract of rat spinal cord, with the prominent major neurofilament subunit indicated, In rodents, NF-H runs at ~200kDa, NF-M at ~145kDa and NF-L at ~68kDa, in larger species such as pig or human, NF-H runs somewhat slower at ~210kDa or 220kDa. (NEFH 抗体)

shows rat mixed neuron/glial cultures stained with mouse monoclonal antibody to neurofilament subunit NF-L MCA-7D1 (green) and ABIN1842223, chicken antibody to neurofilament NF-H. (NEFH 抗体)

2 images

(1 reference)

产品编号 ABIN4956221

发货至: 中国

Quick Overview for NEFH 抗体 (ABIN4956221)

抗原

See all NEFH 抗体

NEFH (Neurofilament, Heavy Polypeptide (NEFH))

适用

人, 大鼠, Pig, Cow

宿主

小鸡

克隆类型

多克隆

标记

This NEFH antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Discover our top product NEFH Primary Antibody

特异性

On western blots CPCA-NF-H recognizes NF-H from all mammalian species tested to date very strongly and shows some weaker reactivity with NF-M. The most immunogenic regions of NF-H and NF-M are the phosphorylated KSP type repeats, and this antibody shows very strong reactivity with these.

产品特性

To raise this particular antibody bovine intermediate filaments were prepared fromspinal cords by the glycerol polymerization method of Delacourte et al., and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis on a Biorad Prepcell. This antibody was generated in chicken by standard procedures and immunoglobulin was extracted from egg yolk. The resulting polyclonal antibody belongs to the IgY subclass. This is the chicken homologue of mammalian IgG and can be used in the same general way, with the caveat that this type of antibody does not bind either Protein A or Protein G.

亚型

IgY
anti-Neurofilament, Heavy Polypeptide (NEFH) antibody
NEFH 适用: 人, 大鼠, 小鼠, 小鸡, Pig IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal NF421 unconjugated

anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 2-251) antibody
NEFH 适用: 人 IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 4F2G12 unconjugated

anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 2-251) antibody
NEFH 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 2C12F3 unconjugated

anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 1-100) antibody
NEFH 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-Neurofilament, Heavy Polypeptide (NEFH) antibody
NEFH 适用: 人, 大鼠, 小鼠, 小鸡, Pig, Cow, 兔, 豚鼠, Gerbil, Cat WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal NE14 unconjugated

anti-Neurofilament, Heavy Polypeptide (NEFH) antibody
NEFH 适用: Cow WB, IF 宿主: 小鸡 Polyclonal unconjugated

anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 968-1020) antibody
NEFH 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 8H10 unconjugated

anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 968-1020) antibody
NEFH 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal unconjugated

anti-Neurofilament, Heavy Polypeptide (NEFH) (AA 21-120) antibody
NEFH 适用: 人, 大鼠, 小鸡 WB, FACS, ELISA, IHC (p), ICC, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Neurofilament, Heavy Polypeptide (NEFH) antibody
NEFH 适用: 人, 大鼠, 小鼠, 小鸡, Pig WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal rNF421 unconjugated Recombinant Antibody

应用备注

The IgY solution has an extremely high titer and can be used at dilutions of at least 1:100,000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:1,000,000 or lower. We supply aliquots of 50 µL, which should be enough for thousands of experiments.

限制

仅限研究用
状态

Liquid

注意事项

Avoid repeat freezing and thawing

储存条件

4 °C/-20 °C
Picchiarelli, Demestre, Zuko, Been, Higelin, Dieterlé, Goy, Mallik, Sellier, Scekic-Zahirovic, Zhang, Rosenbohm, Sijlmans, Aly, Mersmann, Sanjuan-Ruiz, Hübers, Messaddeq, Wagner, van Bakel et al.: "FUS-mediated regulation of acetylcholine receptor transcription at neuromuscular junctions is compromised in amyotrophic lateral sclerosis. ..." in: Nature neuroscience, Vol. 22, Issue 11, pp. 1793-1805, (2020) (PubMed).
抗原

NEFH (Neurofilament, Heavy Polypeptide (NEFH))

背景

Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appears as 10nm diameter fibers of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. They are composed a mixture of subunits which usually include the three neurofilament triplet proteins, known as NF-L, NF-M and NF-H. Neurofilaments may also include smaller amounts of peripherin, α-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable. The HGNC name for this protein is NEFH.