WEE1 抗体 (AA 202-230)
Quick Overview for WEE1 抗体 (AA 202-230) (ABIN3029617)
抗原
See all WEE1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 202-230
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交叉反应 （详细）
- Expected species reactivity: Rat
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纯化方法
- Antigen affinity purified
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免疫原
- A portion of amino acids 202-230 from the mouse protein was used as the immunogen for this Wee1 antibody.
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亚型
- Ig Fraction
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anti-WEE1 Homolog (S. Pombe) (WEE1) (pSer642) antibody
WEE1 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-WEE1 Homolog (S. Pombe) (WEE1) (pSer123) antibodyWEE1 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-WEE1 Homolog (S. Pombe) (WEE1) (AA 289-388) antibodyWEE1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5B6 unconjugated
anti-WEE1 Homolog (S. Pombe) (WEE1) (AA 1-646) antibodyWEE1 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-WEE1 Homolog (S. Pombe) (WEE1) (AA 1-646) antibodyWEE1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-WEE1 Homolog (S. Pombe) (WEE1) (pSer123) antibodyWEE1 适用: 人 IHC (p), DB 宿主: 兔 Polyclonal RB07865 unconjugated
anti-WEE1 Homolog (S. Pombe) (WEE1) (AA 604-634) antibodyWEE1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-WEE1 Homolog (S. Pombe) (WEE1) (pSer123) antibodyWEE1 适用: 人 ELISA, IHC, DB 宿主: 兔 Polyclonal unconjugated
anti-WEE1 Homolog (S. Pombe) (WEE1) (AA 96-130), (Ser123) antibodyWEE1 适用: 人 WB 宿主: 兔 Polyclonal RB57984 unconjugated
anti-WEE1 Homolog (S. Pombe) (WEE1) (AA 289-388) antibodyWEE1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- Titration of the Wee1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- In 1X PBS, pH 7.4, with 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot the Wee1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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- WEE1 (WEE1 Homolog (S. Pombe) (WEE1))
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别名
- Wee1
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背景
- Wee1 may act as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur (By similarity).
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UniProt
- P47810
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途径
- Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase
抗原
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