UCHL3 抗体 (AA 195-225)
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Quick Overview for UCHL3 抗体 (AA 195-225) (ABIN3029426)
抗原
See all UCHL3 (Uchl3) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 195-225
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交叉反应 （详细）
- Expected species reactivity: Mouse, Rat, Bovine, Pig
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纯化方法
- Purified
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免疫原
- A portion of amino acids 195-225 from the human protein was used as the immunogen for this UCHL3 antibody.
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亚型
- Ig Fraction
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anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) (AA 131-230) antibody
Uchl3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4E9 unconjugated
anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) (AA 195-225), (C-Term) antibodyUchl3 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB4187 unconjugated
anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) (AA 1-220) antibodyUchl3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) antibodyUchl3 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) (AA 1-220) antibodyUchl3 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) antibodyUchl3 适用: 人 WB, FACS 宿主: 兔 Monoclonal 24GB1770 unconjugated Recombinant Antibody
anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) (AA 131-230) antibodyUchl3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) (N-Term) antibodyUchl3 适用: 人, 大鼠, 小鼠, Cow, 马, 犬, 兔, 豚鼠, 斑马鱼, Pig, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) (C-Term) antibodyUchl3 适用: 人, 大鼠, 小鼠, Cow, 马, 犬, 兔, Pig, 猴, Hamster IHC, IHC (p), IF, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3) (Internal Region) antibodyUchl3 适用: 人, 大鼠, 小鼠, 犬, 小鸡 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Titration of the UCHL3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- In 1X PBS, pH 7.4, with 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot the UCHL3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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- UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))
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别名
- UCHL3
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背景
- Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54 % identical to that of UCHL1.
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UniProt
- P15374
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途径
- Feeding Behaviour, Positive Regulation of fat Cell Differentiation
抗原
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