RAPTOR 抗体
Quick Overview for RAPTOR 抗体 (ABIN3032434)
抗原
See all RAPTOR 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Purified
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免疫原
- This Raptor antibody was produced from a mouse immunized with recombinant protein.
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亚型
- IgG1 kappa
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anti-RAPTOR (RAPTOR) (pSer863) antibody
RAPTOR 适用: 人 WB, DB, IHC (p) 宿主: 兔 Polyclonal RB24817-24818 unconjugated
anti-RAPTOR (RAPTOR) (AA 101-200) antibodyRAPTOR 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-RAPTOR (RAPTOR) (C-Term) antibodyRAPTOR 适用: 小鼠 WB, IF, EIA 宿主: 兔 Polyclonal unconjugated
anti-RAPTOR (RAPTOR) (pSer863) antibodyRAPTOR 适用: 人 ELISA, DB 宿主: 兔 Polyclonal unconjugated
anti-RAPTOR (RAPTOR) (N-Term) antibodyRAPTOR 适用: 人, 小鼠 WB, IF, EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Titration of the RAPTOR antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. IHC (Paraffin): 1:25,Western blot: 1:500-1:1000
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- In 1X PBS, pH 7.4, with 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot the Raptor antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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- RAPTOR
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别名
- Raptor
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背景
- Raptor is involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals, functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis. [UniProt]
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UniProt
- Q8N122
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途径
- Warburg Effect
抗原
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